1HXE

SERINE PROTEASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-06-22
Detector	RIGAKU RAXIS II
Spacegroup name	C 1 2 1
Unit cell lengths	71.500, 72.300, 73.300
Unit cell angles	90.00, 101.00, 90.00

Refinement procedure

Resolution	7.000 - 2.100
R-factor	0.151 *
Rwork	0.151
RMSD bond length	0.014
RMSD bond angle	0.049
Data reduction software	R-AXIS (II)
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.070 *	2.070 *
Rmerge	0.055 *	0.163 *
Total number of observations	22891 *
Number of reflections	15885	2179 *
Completeness [%]	69.0 *	43 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.3 *		used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	thrombin	5 (mg/ml)	incubated with a 10-fold molar excess of hirugen
2	1	drop	sodium phosphate	0.1 (M)
3	1	drop		0.375 (M)
4	1	drop		1 (mM)
5	1	reservoir	PEG8000	26 (%)