1HX8

CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF DROSOPHILA AP180

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	200
Detector technology	CCD
Collection date	2000-05-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793, 0.9788, 0.9712
Spacegroup name	C 1 2 1
Unit cell lengths	105.632, 106.933, 79.207
Unit cell angles	90.00, 119.30, 90.00

Refinement procedure

Resolution	26.000 - 2.200
R-factor	0.221
Rwork	0.211
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	19.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.076	0.337
Total number of observations	274148 *
Number of reflections	38772 *
<I/σ(I)>	33	5.4
Completeness [%]	99.6	100
Redundancy	7.07	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5 *	298	drop contains protein and reservoir solution in a 1:1 ratio *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	dithiothreitol	1 (mM)
3	1	drop	citrate	50 (mM)
4	1	reservoir	mPEG5000	30 (%)
5	1	reservoir	ammonium sulfate	200 (mM)
6	1	reservoir	MES	100 (mM)