1HX8
CRYSTAL STRUCTURE OF N-TERMINAL DOMAIN OF DROSOPHILA AP180
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2000-05-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793, 0.9788, 0.9712 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.632, 106.933, 79.207 |
| Unit cell angles | 90.00, 119.30, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.211 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 19.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.337 |
| Total number of observations | 274148 * | |
| Number of reflections | 38772 * | |
| <I/σ(I)> | 33 | 5.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.07 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 298 | drop contains protein and reservoir solution in a 1:1 ratio * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | citrate | 50 (mM) | |
| 4 | 1 | reservoir | mPEG5000 | 30 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 6 | 1 | reservoir | MES | 100 (mM) |
