1HWU
STRUCTURE OF PII PROTEIN FROM HERBASPIRILLUM SEROPEDICAE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.38 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.410, 82.320, 100.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.000 - 2.100 |
Rwork | 0.203 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pii |
RMSD bond length | 0.018 |
RMSD bond angle | 0.044 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.057 | 0.322 |
Number of reflections | 36523 | 2415 * |
Completeness [%] | 94.0 * | 95 * |
Redundancy | 3. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | Sodium Cacodylate, Magnesium Acetate, Methylpentadiol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | glycerol | 20 (%) | |
5 | 1 | drop | EDTA | 0.1 (mM) | |
6 | 1 | reservoir | MPD | 30 (%) | |
7 | 1 | reservoir | sodium cacodylate | 0.1 (mM) | pH6.5 |
8 | 1 | reservoir | magnesium acetate | 0.2 (mM) |