1HWO

EBULIN COMPLEXED WITH LACTOSE, TRIGONAL CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Collection date	1999-02-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	74.000, 74.000, 191.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.900
Rwork	0.237
R-free	0.33900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ebulin model (PDB 1HWM) orthorhombic crystal form
RMSD bond length	0.003
RMSD bond angle	27.685 *
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.087	0.346
Number of reflections	12522
<I/σ(I)>	11.5
Completeness [%]	97.7	91.1
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	25 *	1.1 M Na,K Tartrate, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	Na, K tartrate	1.1 (M)
3	1	reservoir	HEPES	100 (mM)