1HWN

EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Collection date	1999-10-16
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	73.600, 73.600, 190.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.264
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ebulin trigonal crystal form model complexed with lactose
RMSD bond length	0.003
RMSD bond angle	28.304 *
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.089	0.467
Number of reflections	15454
<I/σ(I)>	11.6
Completeness [%]	97.7	98.6
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	25 *	1.1 M Na,K Tartrate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	Na, K tartrate	1.1 (M)
3	1	reservoir	HEPES	100 (mM)