1HW7
HSP33, HEAT SHOCK PROTEIN WITH REDOX-REGULATED CHAPERONE ACTIVITY
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 77.180, 77.180, 199.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.229 * |
Rwork | 0.230 |
R-free | 0.25670 |
Structure solution method | MAD |
Starting model (for MR) | Structure phased by three-wavelength MAD experiment of AUCN-derived crystals and refined to 2.4 A. |
RMSD bond length | 0.015 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.048 | 0.323 |
Total number of observations | 444763 * | |
Number of reflections | 18629 | |
<I/σ(I)> | 45.7 | 14 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 23.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 22 * | Drops contained 2 microlitres of protein solution (10mg/ml in 20mM KCL, 40mM HEPES pH 7.5) supplemented with 2mM DTT and 0.3 mM ZnCl2 and 2 microlitres of precipitant (1.5 M ammonium sulfate, 10% dioxane, 0.1 M MES pH 6.8) and were equilibrated against 1ml of the same precipitant., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | HEPES | 40 (mM) | |
4 | 1 | drop | dithiothreitol | 20 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
6 | 1 | reservoir | dioxane | 10 (%) | |
7 | 1 | reservoir | MES | 0.1 (M) |