1HW5
THE CAP/CRP VARIANT T127L/S128A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-03-12 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.100, 93.100, 104.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.820 |
| R-factor | 0.228 |
| Rwork | 0.227 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GAP |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.025 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.088 | 0.088 |
| Total number of observations | 177444 * | |
| Number of reflections | 38054 * | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 97.7 * | 98 |
| Redundancy | 4.7 * | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | PEG 4K, isoprotanol, cAMP, HEPES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.8 (mg/ml) | |
| 10 | 1 | reservoir | HEPES | 0.1 (M) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | EDTA | 0.2 (mM) | |
| 4 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 5 | 1 | drop | 0.5 (M) | ||
| 6 | 1 | drop | dithiothreitol | 0.2 (mM) | |
| 7 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 8 | 1 | reservoir | isopropanol | 7 (%(v/v)) | |
| 9 | 1 | reservoir | cAMP | 10 (mM) |
