1HW5
THE CAP/CRP VARIANT T127L/S128A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1998-03-12 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.100, 93.100, 104.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.820 |
R-factor | 0.228 |
Rwork | 0.227 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3GAP |
RMSD bond length | 0.007 |
RMSD bond angle | 0.025 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.088 | 0.088 |
Total number of observations | 177444 * | |
Number of reflections | 38054 * | |
<I/σ(I)> | 12 | |
Completeness [%] | 97.7 * | 98 |
Redundancy | 4.7 * | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | PEG 4K, isoprotanol, cAMP, HEPES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10.8 (mg/ml) | |
10 | 1 | reservoir | HEPES | 0.1 (M) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | EDTA | 0.2 (mM) | |
4 | 1 | drop | glycerol | 5 (%(v/v)) | |
5 | 1 | drop | 0.5 (M) | ||
6 | 1 | drop | dithiothreitol | 0.2 (mM) | |
7 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
8 | 1 | reservoir | isopropanol | 7 (%(v/v)) | |
9 | 1 | reservoir | cAMP | 10 (mM) |