1HVX
BACILLUS STEAROTHERMOPHILUS ALPHA-AMYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-01-01 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.550, 93.119, 53.275 |
| Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.156 * |
| Rwork | 0.154 |
| R-free | 0.19660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.202 |
| Number of reflections | 29849 | |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 89.0 | 73 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 293 | SODIUM ACETATE, CALCIUM CLORIDE, 2-PROPANOL, ACARBOSE , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium acetate | 35 (mM) | pH4.6 |
| 2 | 1 | reservoir | 35 (mM) | ||
| 3 | 1 | reservoir | 2-propanol | 6.25 (%(v/v)) | |
| 4 | 1 | reservoir | acarbose | 1.23 (%(w/v)) | |
| 5 | 1 | drop | protein | 10 (mg/ml) |
