1HVV

SELF-ASSOCIATION OF THE H3 REGION OF SYNTAXIN 1A: IMPLICATIONS FOR SNARE COMPLEX ASSEMBLY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-04-03
Detector	RIGAKU RAXIS II
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	88.320, 88.320, 115.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.238
Rwork	0.238
R-free	0.27300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	45.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.054 *	0.313
Number of reflections	20870
<I/σ(I)>	19.8
Completeness [%]	99.2	99.1
Redundancy	4.4 *	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4 *	25 *	sodium potassium tartrate, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		300 (mM)
3	1	drop	Tris	15 (mM)
4	1	reservoir	sodium potassium tartrate	750 (mM)
5	1	reservoir	sodium acetate	100 (mM)