1HVQ

CRYSTAL STRUCTURES OF HEVAMINE, A PLANT DEFENCE PROTEIN WITH CHITINASE AND LYSOZYME ACTIVITY, AND ITS COMPLEX WITH AN INHIBITOR

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1993-11-15
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	52.300, 57.360, 82.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400 *
R-factor	0.144
Rwork	0.144
R-free	0.24400
RMSD bond length	0.015
RMSD bond angle	3.100
Data reduction software	MADNES
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	28.000 *
High resolution limit [Å]	2.400 *
Rmerge	0.082
Number of reflections	9453
Completeness [%]	92.0
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	35-65 (%sat)
2	1	reservoir	sodium chloride	1.7-3.4 (M)	precipitant