1HVG

STRUCTURAL AND ELECTROPHYSIOLOGICAL ANALYSIS OF ANNEXIN V MUTANTS. MUTAGENESIS OF HUMAN ANNEXIN V, AN IN VITRO VOLTAGE-GATED CALCIUM CHANNEL, PROVIDES INFORMATION ABOUT THE STRUCTURAL FEATURES OF THE ION PATHWAY, THE VOLTAGE SENSOR AND THE ION SELECTIVITY FILTER

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	156.900, 156.900, 36.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 * - 3.000
R-factor	0.199
Rwork	0.199
RMSD bond length	0.014
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	3.000	3.000 *
Rmerge	0.073 *
Total number of observations	17631 *
Number of reflections	5103
Completeness [%]	75.0	44.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MOPS	3 (mM)
2	1	drop	protein	9 (mg/ml)
3	1	drop		20 (mM)
4	1	reservoir	ammonium sulfate	2.1 (M)
5	1	reservoir	Tris-chloride	0.1 (M)