1HV9
STRUCTURE OF E. COLI GLMU: ANALYSIS OF PYROPHOSPHORYLASE AND ACETYLTRANSFERASE ACTIVE SITES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.500, 104.500, 648.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.250 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24800 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.250 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.136 * | 0.233 |
| Total number of observations | 495967 * | |
| Number of reflections | 91622 * | |
| <I/σ(I)> | 9.7 | 1.62 |
| Completeness [%] | 98.0 * | 82.2 |
| Redundancy | 5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | MES, ammonium sulfate, magnesium chloride, cobalt chloride, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.3 (mg/ml) | |
| 2 | 1 | drop | UDP-GlcNAc | 14 (mM) | |
| 3 | 1 | drop | 28 (mM) | ||
| 4 | 1 | drop | 19 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.65 (M) | |
| 6 | 1 | reservoir | MES | 50 (mM) | |
| 7 | 1 | reservoir | 2-10 (mM) |
