1HV8
CRYSTAL STRUCTURE OF A DEAD BOX PROTEIN FROM THE HYPERTHERMOPHILE METHANOCOCCUS JANNASCHII
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.9793, 0.9611 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.132, 131.450, 131.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.27 * |
| Rwork | 0.270 |
| R-free | 0.31600 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (+ DM) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.097 | 0.419 |
| Total number of observations | 322686 * | |
| Number of reflections | 20100 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 303 | ammonium sulfate, acetate,tris, DTT, EDTA, NaN3, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | |
| 3 | 1 | drop | 0.02 (%(w/v)) | ||
| 4 | 1 | drop | ammonium sulfate | 500 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | drop | dithiothreitol | 5 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 57-62 (%sat) | |
| 9 | 1 | reservoir | sodium acetate | 100 (mM) |
