1HUV
CRYSTAL STRUCTURE OF A SOLUBLE MUTANT OF THE MEMBRANE-ASSOCIATED (S)-MANDELATE DEHYDROGENASE FROM PSEUDOMONAS PUTIDA AT 2.15A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | I 4 |
| Unit cell lengths | 99.590, 99.590, 87.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.420 - 2.150 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT + SIR |
| Starting model (for MR) | 1gox |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.092 * | 0.362 * |
| Number of reflections | 28143 | |
| <I/σ(I)> | 21.7 | 6.3 |
| Completeness [%] | 98.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | MES, ammonium sulfate, ethylene glycerol, FMN, NaCl, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | MES | 0.2 (M) | |
| 3 | 1 | drop | ammonium sulfate | 0.75 (%) | |
| 4 | 1 | drop | ethylene glycol | 10 (%) | |
| 5 | 1 | drop | FMN | 0.020 (mM) | |
| 6 | 1 | reservoir | 4.0 (M) | 1ml |
