1HUU
DNA-BINDING PROTEIN HU FROM BACILLUS STEAROTHERMOPHILUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 293 |
Detector technology | FILM |
Collection date | 1983-07 |
Detector | FILM |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 65.500, 37.300, 65.500 |
Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21300 |
Structure solution method | SINGLE ISOMORPHOUS REPLACEMENT PLUS ANOMALOUS (URANYL) AND NCS AVERAGING ON THREE MOLECULES |
RMSD bond length | 0.011 |
RMSD bond angle | 1.530 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.190 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.100 |
Number of reflections | 18328 | |
<I/σ(I)> | 10 | 2.5 |
Completeness [%] | 89.0 | 80 |
Redundancy | 4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 8 (mg/ml) | |
2 | 1 | 1 | phosphate | 25 (mM) | |
3 | 1 | 2 | MPD | 35 (%) |