1HUU
DNA-BINDING PROTEIN HU FROM BACILLUS STEAROTHERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 293 |
| Detector technology | FILM |
| Collection date | 1983-07 |
| Detector | FILM |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 65.500, 37.300, 65.500 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21300 |
| Structure solution method | SINGLE ISOMORPHOUS REPLACEMENT PLUS ANOMALOUS (URANYL) AND NCS AVERAGING ON THREE MOLECULES |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.530 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.190 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.100 |
| Number of reflections | 18328 | |
| <I/σ(I)> | 10 | 2.5 |
| Completeness [%] | 89.0 | 80 |
| Redundancy | 4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 8 (mg/ml) | |
| 2 | 1 | 1 | phosphate | 25 (mM) | |
| 3 | 1 | 2 | MPD | 35 (%) |
