1HUP
HUMAN MANNOSE BINDING PROTEIN CARBOHYDRATE RECOGNITION DOMAIN TRIMERIZES THROUGH A TRIPLE ALPHA-HELICAL COILED-COIL
Experimental procedure
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 76.680, 76.680, 58.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.193 * |
| Rwork | 0.199 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.100 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.088 |
| Total number of observations | 22595 * |
| Number of reflections | 6978 |
| Completeness [%] | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | Chang, C.Y., (1994) J.Mol.Biol., 241, 125. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | reservoir | Tris-maleic acid | 100 (mM) | |
| 3 | 1 | reservoir | PEG600 | 18 (%) | |
| 4 | 1 | reservoir | 12 (mg/ml) | ||
| 5 | 1 | reservoir | 10 (mM) |
