1HUH

DIFFERENCES IN ANIONIC INHIBITION OF HUMAN CARBONIC ANHYDRASE I REVEALED FROM THE STRUCTURES OF IODIDE AND GOLD CYANIDE INHIBITOR COMPLEXES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	81.800, 75.200, 37.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.166
RMSD bond length	0.017 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.167 *
Number of reflections	10061 *	276 *
Completeness [%]	82.4 *	23.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	8.7 *	4 *	referred to 'Kannan,K.K.', (1972) 'J. Mol. Biol.', 63, 601-604 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2.5 (M)
2	1	1	Tris-HCl	0.05 (M)