1HUF

CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-11-05
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	48.063, 120.651, 49.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.000
R-factor	0.1921
Rwork	0.189
R-free	0.26000
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	5.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.080
High resolution limit [Å]	2.000	2.010
Rmerge	0.072	0.340
Number of reflections	8860
<I/σ(I)>	15.3	3.6
Completeness [%]	83.3	55 *
Redundancy	4.01	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	293	PEG4000, bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	12 (%)
2	1	reservoir	Tris-acetate	100 (mM)	or sodium-bicine