1HUF
CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.063, 120.651, 49.036 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.000 |
| R-factor | 0.1921 |
| Rwork | 0.189 |
| R-free | 0.26000 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 5.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.010 |
| Rmerge | 0.072 | 0.340 |
| Number of reflections | 8860 | |
| <I/σ(I)> | 15.3 | 3.6 |
| Completeness [%] | 83.3 | 55 * |
| Redundancy | 4.01 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 293 | PEG4000, bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 12 (%) | |
| 2 | 1 | reservoir | Tris-acetate | 100 (mM) | or sodium-bicine |
