1HTV
CRYSTAL STRUCTURE OF DESTRIPEPTIDE (B28-B30) INSULIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.810, 51.550, 100.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | crystal structure of 2ZN Insulin |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.189 |
| Total number of observations | 481068 * | |
| Number of reflections | 20516 * | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 97.5 * | 91.3 |
| Redundancy | 18 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | sodium citrate, dimethylformamide, zinc acetate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | 0.02 (M) | ||
| 3 | 1 | reservoir | trisodium citrate | 0.1 (M) | |
| 4 | 1 | reservoir | DMF | 15 (%(v/v)) | |
| 5 | 1 | reservoir | zinc acetate | 0.13 (%(w/v)) | |
| 6 | 1 | reservoir | 1 (M) |
