1HTT

HISTIDYL-TRNA SYNTHETASE

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	275
Detector technology	IMAGE PLATE
Collection date	1994-02-09
Detector	MAR scanner 300 mm plate
Spacegroup name	P 1
Unit cell lengths	61.400, 110.700, 108.700
Unit cell angles	115.00, 97.40, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.246
Rwork	0.246
R-free	0.32700
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT, DENSITY AVERAGING
RMSD bond length	0.015
RMSD bond angle	25.072 *
Data reduction software	MARXDS
Data scaling software	Agrovata
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.057	0.231
Number of reflections	54820
<I/σ(I)>	10.1	2.8
Completeness [%]	69.4	55.7
Redundancy	1.7	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.4		pH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein complex	10 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir		0.5 (M)
4	1	reservoir	PEG6000	10 (%)