1HTP

REFINED STRUCTURES AT 2 ANGSTROMS AND 2.2 ANGSTROMS OF THE TWO FORMS OF THE H-PROTEIN, A LIPOAMIDE-CONTAINING PROTEIN OF THE GLYCINE DECARBOXYLASE COMPLEX

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	61.340, 55.380, 33.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.185
Rwork	0.185
RMSD bond length	0.014
RMSD bond angle	2.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.054 *
Total number of observations	14236 *
Number of reflections	5684
Completeness [%]	91.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2 *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	H-Proox	7-14 (mg/ml)	can be replaced with 3-5mg/ml H-ProMet
2	1	drop	TrisMaleate	0.1 (M)
3	1	reservoir	ammonium sulfate	2 (M)