1HTB

CRYSTALLIZATION OF HUMAN BETA3 ALCOHOL DEHYDROGENASE (10 MG/ML) IN 100 MM SODIUM PHOSPHATE (PH 7.5), 7.5 MM NAD+ AND 1 MM 4-IODOPYRAZOLE AT 25 C

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-02-14
Detector	RIGAKU
Spacegroup name	P 1
Unit cell lengths	54.030, 44.430, 92.740
Unit cell angles	92.71, 103.17, 69.15

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.175
Rwork	0.175
R-free	0.24300
RMSD bond length	0.008
RMSD bond angle	25.220 *
Data reduction software	R-AXIS (IIC (HAGASHI/RIGAKU))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.970
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.100
Total number of observations	53103 *
Number of reflections	26989
Completeness [%]	83.7	70 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	recombinant human beta3	10 (mg/ml)
2	1	drop	sodium phosphate	100 (mM)
3	1	drop	NAD+	7.5 (mM)
4	1	drop	4-iodopyrazole	1 (mM)
5	1	drop	PEG8000	16.5 (%(w/v))