1HRU

THE STRUCTURE OF THE YRDC GENE PRODUCT FROM E.COLI

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-26
Detector	CUSTOM-MADE
Wavelength(s)	0.9795, 0.9794, 0.9465
Spacegroup name	P 1 21 1
Unit cell lengths	60.500, 71.650, 55.280
Unit cell angles	90.00, 103.25, 90.00

Refinement procedure

Resolution	22.750 - 2.000
R-factor	0.202
Rwork	0.202
R-free	0.21400
Structure solution method	MAD
RMSD bond length	0.009 *
RMSD bond angle	1.500 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.062	0.486
Total number of observations	230966 *
Number of reflections	62780
<I/σ(I)>	17.6	2.6
Completeness [%]	96.2	99.8
Redundancy	3.68	3.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	sodium potassium phosphate	0.6 (M)