1HRH

CRYSTAL STRUCTURE OF THE RIBONUCLEASE H DOMAIN OF HIV-1 REVERSE TRANSCRIPTASE

Experimental procedure

Spacegroup name	P 31
Unit cell lengths	51.900, 51.900, 114.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.400
R-factor	0.2
RMSD bond length	0.022
RMSD bond angle	0.051
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.045 *	0.198 *
Total number of observations	32084 *
Number of reflections	13047 *	2521 *
Completeness [%]	93.1 *	90.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	25 (mM)
3	1	reservoir	sodium potassium tartrate	0.15 (M)
4	1	reservoir	PEG8000	20 (%)
5	1	reservoir	sodium citrate	0.1 (M)