1HR2
CRYSTAL STRUCTURE ANALYSIS OF A MUTANT P4-P6 DOMAIN (DELC209) OF TETRAHYMENA THEMOPHILA GROUP I INTRON.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-03-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.300, 125.400, 145.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.250 |
Rwork | 0.244 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gid |
RMSD bond length | 0.011 |
RMSD bond angle | 1.510 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.051 | 0.578 |
Number of reflections | 64900 | |
<I/σ(I)> | 22.7 | 2.4 |
Completeness [%] | 98.0 | 99.3 |
Redundancy | 4.8 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 30 * | drop is set up using 2:8:5 ratio of solutions a, b and c * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | sodium cacodylate | ||
4 | 1 | 1 | spermine | ||
5 | 1 | 1 | NaCl | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium cacodylate | 250 (mM) | solution a |
10 | 1 | reservoir | MPD | 17 (%(w/v)) | |
11 | 1 | reservoir | 170 (mM) | ||
2 | 1 | drop | 200 (mM) | solution a | |
3 | 1 | drop | spermine | 2.5 (mM) | solution a |
4 | 1 | drop | P4-P6 RNA | 4 (mg/ml) | solution b |
5 | 1 | drop | potassium cacodylate | 63 (mM) | solution b |
6 | 1 | drop | 13 (mM) | solution b | |
7 | 1 | drop | cobalt hexammine | 0.16 (mM) | solution b |
8 | 1 | drop | EDTA | 0.1 (mM) | solution b |
9 | 1 | drop | MPD | 20-25 (%(w/v)) | solution c |