1HQL

The xenograft antigen in complex with the B4 isolectin of Griffonia simplicifolia lectin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	111.210, 51.280, 77.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.200
R-factor	0.233 *
Rwork	0.233
R-free	0.25400
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.142	0.416
Number of reflections	22043
<I/σ(I)>	16	4.6
Completeness [%]	96.7	83.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tempel, W., (2001) Acta Crystallogr., D57, 1639. *
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tempel, W., (2001) Acta Crystallogr., D57, 1639. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	detergent NP-40	0.02 (%(w/v))
3	1	reservoir	PEG4000	11 (%(w/v))
4	1	reservoir	MPD	8 (%(v/v))
5	1	reservoir	DMSO	6 (%(v/v))
6	1	reservoir	HEPES	0.1 (M)	pH7.5