1HQH
CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH NOR-N-HYDROXY-L-ARGININE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-28 |
| Spacegroup name | P 32 |
| Unit cell lengths | 88.000, 88.000, 112.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.045 | 0.319 |
| Total number of observations | 55538 * | |
| Number of reflections | 22632 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 8000, BICINE, manganese chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Bicine | 50 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 5 | 1 | reservoir | Bicine | 50 (mM) | |
| 6 | 1 | reservoir | 1 (mM) | ||
| 7 | 1 | reservoir | sodium azide | 0.05 (%(w/v)) |
