1HQ3

CRYSTAL STRUCTURE OF THE HISTONE-CORE-OCTAMER IN KCL/PHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-03-01
Detector	MARRESEARCH
Wavelength(s)	1.488
Spacegroup name	P 65
Unit cell lengths	158.560, 158.560, 102.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.150
R-factor	0.214 *
Rwork	0.214
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	histone octamer PDB entry 1HIO
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.250
High resolution limit [Å]	2.150	2.150
Rmerge	0.071	0.331
Total number of observations	169058 *
Number of reflections	74806
Completeness [%]	97.4	97.1
Redundancy	2.26	2.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.9 *	277 *	2.0M KCL, 1.35M phosphate, pH 6.6, pH 6.7, MICRODIALYSIS
1	MICRODIALYSIS	6.9 *	277 *	2.0M KCL, 1.35M phosphate, pH 6.6, pH 6.7, MICRODIALYSIS

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		0.475 (M)
2	1	1		0.475 (M)
3	1	1	protein	20 (mg/ml)
4	1	2		2 (M)
5	1	2		0.675 (M)
6	1	2		0.675 (M)