1HQ3
CRYSTAL STRUCTURE OF THE HISTONE-CORE-OCTAMER IN KCL/PHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.488 |
Spacegroup name | P 65 |
Unit cell lengths | 158.560, 158.560, 102.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.25320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | histone octamer PDB entry 1HIO |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.071 | 0.331 |
Total number of observations | 169058 * | |
Number of reflections | 74806 | |
Completeness [%] | 97.4 | 97.1 |
Redundancy | 2.26 | 2.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.9 * | 277 * | 2.0M KCL, 1.35M phosphate, pH 6.6, pH 6.7, MICRODIALYSIS |
1 | MICRODIALYSIS | 6.9 * | 277 * | 2.0M KCL, 1.35M phosphate, pH 6.6, pH 6.7, MICRODIALYSIS |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | 0.475 (M) | ||
2 | 1 | 1 | 0.475 (M) | ||
3 | 1 | 1 | protein | 20 (mg/ml) | |
4 | 1 | 2 | 2 (M) | ||
5 | 1 | 2 | 0.675 (M) | ||
6 | 1 | 2 | 0.675 (M) |