1HQ1
STRUCTURAL AND ENERGETIC ANALYSIS OF RNA RECOGNITION BY A UNIVERSALLY CONSERVED PROTEIN FROM THE SIGNAL RECOGNITION PARTICLE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-20 |
| Wavelength(s) | 1.039177, 1.039988, 0.999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.555, 78.337, 32.872 |
| Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.520 |
| R-factor | 0.151 |
| Rwork | 0.151 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dul |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.070 | 0.490 |
| Number of reflections | 51967 | |
| <I/σ(I)> | 35.3 | 1.6 |
| Completeness [%] | 98.2 | 89.3 |
| Redundancy | 10.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | Na-MES, isopropanol, potassium chloride, magnesium chloride, MPD, pH 5.6, EVAPORATION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na-MES | ||
| 2 | 1 | 1 | isopropanol | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | isopropanol | 10 (%) | |
| 2 | 1 | reservoir | Na-Mes | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 12.5 (mM) |
