1HPM

HOW POTASSIUM AFFECTS THE ACTIVITY OF THE MOLECULAR CHAPERONE HSC70. II. POTASSIUM BINDS SPECIFICALLY IN THE ATPASE ACTIVE SITE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	143.900, 63.700, 46.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.700
R-factor	0.205
Rwork	0.205
RMSD bond length	0.007
RMSD bond angle	1.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	1.700
Rmerge	0.054 *
Total number of observations	174376 *
Number of reflections	39253 *
Completeness [%]	82.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	4 *	DeLuca-Flaherty, C., (1988) J. Mol. Biol., 200, 749. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	V8-generated fragment	5-10 (mg/ml)
2	1	reservoir		1 (mM)
3	1	reservoir	ADP	1 (mM)
4	1	reservoir		1 (M)
5	1	reservoir	PEG8000	20 (%)
6	1	reservoir	MES	50 (mM)