1HPI

MOLECULAR STRUCTURE OF THE OXIDIZED HIGH-POTENTIAL IRON-SULFUR PROTEIN ISOLATED FROM ECTOTHIORHODOSPIRA VACUOLATA

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	58.400, 64.700, 39.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.800
R-factor	0.163
RMSD bond length	0.007
RMSD bond angle	15.600 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.049 *	0.095 *
Total number of observations	19816 *
Number of reflections	7028 *	1134 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			in the presence of atmospheric oxygen *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.2 (M)
2	1	reservoir	sodium/potassium phosphate	50 (mM)
3	1	reservoir		5 (mM)
4	1	drop	protein	20-40 (mg/ml)