1HPG

A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	77.260, 36.290, 51.220
Unit cell angles	90.00, 101.80, 90.00

Refinement procedure

Resolution	? - 2.000 *
R-factor	0.17 *
Rwork	0.181
RMSD bond length	0.014
RMSD bond angle	3.180 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800 *
Rmerge	0.083 *
Total number of observations	90655 *
Number of reflections	22913
Completeness [%]	94.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	33 (mg/ml)
2	1	drop		0.2 (M)
3	1	drop	HEPES	0.1 (M)
4	1	reservoir	sodium citrate	50 (%sat)