1HPB

THE BACTERIAL PERIPLASMIC HISTIDINE-BINDING PROTEIN: STRUCTURE(SLASH)FUNCTION ANALYSIS OF THE LIGAND-BINDING SITE AND COMPARISON WITH RELATED PROTEINS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	39.260, 66.170, 88.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.500
R-factor	0.192
Rwork	0.192
RMSD bond length	0.020
RMSD bond angle	3.640
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.082 *
Number of reflections	6525 *
Completeness [%]	77.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.3 (M)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir	PEG4000	25 (%)