1HOT

GLUCOSAMINE 6-PHOSPHATE DEAMINASE COMPLEXED WITH THE ALLOSTERIC ACTIVATOR N-ACETYL-GLUCOSAMINE-6-PHOSPHATE

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	125.710, 125.710, 222.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.165
Rwork	0.165
RMSD bond length	0.005
RMSD bond angle	23.900 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Rmerge	0.051 *
Number of reflections	20036 *
Completeness [%]	88.1 *
Redundancy	1.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Na/K phosphate
2	1	reservoir	GlcNAc6P	0.45 (mM)