1HNY

The structure of human pancreatic alpha-amylase at 1.8 angstroms resolution and comparisons with related enzymes

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	53.040, 74.800, 137.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.174
Rwork	0.174
RMSD bond length	0.016
RMSD bond angle	0.036 *
Data reduction software	MSC
Phasing software	X-PLOR (2.1)
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.600
High resolution limit [Å]	1.800	1.800 *
Rmerge	0.055
Number of reflections	42316
Completeness [%]	82.1	63.1 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		1 (mM)
3	1	drop	cacodylate	100 (mM)
4	1	reservoir	MPD	60 (%)