1HNW
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH TETRACYCLINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-25 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 401.158, 401.158, 176.944 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.750 - 3.400 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1FJF |
| RMSD bond length | 0.007 |
| RMSD bond angle | 28.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.178 * | 0.411 * |
| Total number of observations | 704712 * | |
| Number of reflections | 193543 | |
| <I/σ(I)> | 4.1 | 1.55 |
| Completeness [%] | 97.4 | 95.4 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 277 | Wimberly, B.T., (2000) Nature, 407, 327. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4Cl | ||
| 10 | 1 | 2 | magnesium acetate | ||
| 11 | 1 | 2 | sodium cacodylate | ||
| 12 | 1 | 2 | MPD | ||
| 13 | 1 | 3 | tetracycline | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | magnesium acetate | ||
| 5 | 1 | 1 | sodium cacodylate | ||
| 6 | 1 | 1 | MPD | ||
| 7 | 1 | 2 | NH4Cl | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | CaCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 4 | 1 | 2-mercaptoethanol | 6 (mM) | ||
| 5 | 1 | potassium cacodylate | 0.1 (M ) | or 0.1M MES |
