1HNL

CRYSTAL STRUCTURE OF A GLUTATHIONYLATED HUMAN LYSOZYME: A FOLDING INTERMEDIATE MIMIC IN THE FORMATION OF A DISULFIDE BOND

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	57.190, 60.960, 33.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.125
RMSD bond length	0.017
RMSD bond angle	0.040
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.760 *
Rmerge	0.067 *
Total number of observations	30257 *
Number of reflections	9501
Completeness [%]	84.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6 *	293 *	using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2		2.5 (M)
3	1	2	sodium phosphate	30 (mM)