1HN1
E. COLI (LAC Z) BETA-GALACTOSIDASE (ORTHORHOMBIC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 153.900, 171.400, 204.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| Rwork | 0.148 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dp0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.678 |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.292 |
| Number of reflections | 103758 | |
| <I/σ(I)> | 7.2 | 2.3 |
| Completeness [%] | 95.1 | 98.5 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 288 | Juers, D.H., (2000) Protein Sci., 9, 1685., used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | Bis-Tris | 100 (mM) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) |
