1HMY

CRYSTAL STRUCTURE OF THE HHAI DNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYL-L-METHIONINE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	55.300, 72.700, 91.000
Unit cell angles	90.00, 102.50, 90.00

Refinement procedure

Resolution	8.000 * - 2.500
R-factor	0.2
Rwork	0.200
RMSD bond length	0.012
RMSD bond angle	1.820
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.072 *
Total number of observations	43292 *
Number of reflections	20981 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6 *	16 *	referred to Biochemistry 31.8648-8653 1992 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	FPLC buffer A
3	1	drop		0.2 (M)
4	1	reservoir	PEG4000	25 (%(w/v))
5	1	reservoir	ammonium sulfate	50 (mM)
6	1	reservoir	citrate	100 (M)