1HMK
RECOMBINANT GOAT ALPHA-LACTALBUMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 282 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-01 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 44.850, 88.940, 32.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.191 * |
| Rwork | 0.198 |
| R-free | 0.27800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BABOON ALPHA-LACTALBUMIN |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.554 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.069 | 0.309 |
| Number of reflections | 12533 | |
| Completeness [%] | 92.5 | 94.6 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20 * | pH 6.0, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 1.0 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 16-20 (%) | |
| 4 | 1 | reservoir | potassium phosphate | 0.05 (M) |
