1HMH
THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 89.740, 89.740, 185.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.600 |
R-factor | 0.257 |
Rwork | 0.257 |
R-free | 0.30500 |
RMSD bond length | 0.010 |
RMSD bond angle | 10.300 * |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.600 |
Number of reflections | 26816 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | NH4 SULFATE | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | SPERMINE | ||
5 | 1 | 1 | CACODYLATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 10 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 1.9-2.2 (M) | or 1.5-2.2 M LiSO4 |
4 | 1 | reservoir | 0-100 (mM) | ||
5 | 1 | reservoir | spermine | 0-2 (mM) | |
6 | 1 | reservoir | cacodylate | 10 (mM) |