1HMH
THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.740, 89.740, 185.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.30500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 10.300 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 26816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NH4 SULFATE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.9-2.2 (M) | or 1.5-2.2 M LiSO4 |
| 4 | 1 | reservoir | 0-100 (mM) | ||
| 5 | 1 | reservoir | spermine | 0-2 (mM) | |
| 6 | 1 | reservoir | cacodylate | 10 (mM) |
