1HM8
CRYSTAL STRUCTURE OF S.PNEUMONIAE N-ACETYLGLUCOSAMINE-1-PHOSPHATE URIDYLTRANSFERASE, GLMU, BOUND TO ACETYL COENZYME A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9326 |
Spacegroup name | H 3 |
Unit cell lengths | 92.459, 92.459, 279.562 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.630 - 2.500 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.24300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 25.100 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.024 * | 0.069 |
Total number of observations | 73418 * | |
Number of reflections | 29218 | |
<I/σ(I)> | 23.1 | 10.8 |
Completeness [%] | 94.7 | 96.6 |
Redundancy | 2.5 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | 18 % PEG 400 (w/w) 300 mM CaCl2 50 mM NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 26 (%(v/v)) | |
3 | 1 | reservoir | 50 (mM) | ||
4 | 1 | reservoir | 300 (mM) | pH8.0 |