1HLF
BINDING OF GLUCOPYRANOSYLIDENE-SPIRO-THIOHYDANTOIN TO GLYCOGEN PHOSPHORYLASE B: KINETIC AND CRYSTALLOGRAPHIC STUD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.787, 128.787, 116.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.060 - 2.260 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22100 |
| Structure solution method | OTHER |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.041 | 0.158 |
| Total number of observations | 255617 * | |
| Number of reflections | 45104 | |
| Completeness [%] | 97.4 | 99.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | 16 * | Oikonomakos, N.G., (1985) Biochim.Biophys.Acta., 832, 248. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | phosphorylase b | 27.5 (mg/ml) | |
| 2 | 1 | 1 | Bes | 10 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 2.9 (mM) | |
| 4 | 1 | 1 | EDTA | 0.1 (mM) | |
| 5 | 1 | 1 | spermine | 1.1 (mM) |
