1HLD

STRUCTURES OF HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED WITH NAD+ AND SUBSTITUTED BENZYL ALCOHOLS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	51.100, 180.700, 44.300
Unit cell angles	90.00, 108.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.183
Rwork	0.183
RMSD bond length	0.010
RMSD bond angle	1.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.055 *
Total number of observations	65557 *
Number of reflections	37152 *
Completeness [%]	84.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *	3-7 *	Plapp, B. V., (1978) J.Mol.Biol., 122, 23. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10 (mg/ml)
2	1	1	NAD+	0.5 (mg/ml)
3	1	1		0.5 (mg/ml)
4	1	1	TES-NH3	0.05 (M)