1HKW
MYCOBACTERIUM DIAMINOPIMELATE DICARBOXYLASE (LysA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2002-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.560, 111.560, 237.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 1.699 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.028 | 0.136 |
| Total number of observations | 813664 * | |
| Number of reflections | 35928 * | 2637 * |
| <I/σ(I)> | 28.2 | 7.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 22.6 | 22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 * | 18 * | 4 UL HANGING DROPS (2 UL OF DAPDC 10 MG/ML CONTAINING 5MM OF LYSINE COMBINED WITH 2 UL OF WELL SOLUTION) WERE EQUILIBRATED AGAINST 500 UL OF WELL SOLUTION (24% PEG-MME 5K, 0.1M MES BUFFER PH6.3 AND 60 MM AMMONIUM SULFATE), pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | lysine | 5 (mM) | |
| 3 | 1 | reservoir | PEG5000 MME | 24 (%) | |
| 4 | 1 | reservoir | MES | 0.1 (M) | pH6.3 |
| 5 | 1 | reservoir | ammonium sulfate | 60 (mM) |
