1HKQ
PPS10 plasmid DNA replication initiator protein RepA. Replication inactive, dimeric N-terminal domain.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0090, 1.0120 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.866, 55.533, 96.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.890 - 2.750 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.081 | 0.320 |
| Total number of observations | 44839 * | |
| Number of reflections | 8343 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 97.7 | 97.8 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | VAPOUR DIFFUSION (HANGING DROPS), WELLS: 500 MICROLITRES (14% PEG4000, 6% MPD), DROPS: 5 MICROLITRES PROTEIN (5 MG/ML IN 50 MM K2HPO4/ KH2PO4, PH= 6.2),5 MICROLITRES WELL SOL. PLUS 1 MICROLITRE OF P-CL-MERCURIBENZOATE (=1 MM IN WATER), THIN LARGE CRYSTAL PRISMS APPEAR AT 295 DEGREES KELVIN IN 1 MONTH., pH 6.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
| 2 | 1 | reservoir | MPD | 6 (%(v/v)) | |
| 3 | 1 | drop | protein | 5 (mg/ml) | |
| 4 | 1 | drop | potassium phosphate | 50 (mM) | pH6.2 |
