1HKH

unligated gamma lactamase from an Aureobacterium species

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	MARRESEARCH
Spacegroup name	F 2 3
Unit cell lengths	240.874, 240.874, 240.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.000 - 1.730
Rwork	0.144
R-free	0.15800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bro
RMSD bond length	0.013 *
RMSD bond angle	1.347 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	11.000	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.108	0.480
Number of reflections	119247
<I/σ(I)>	24.76	5.23
Completeness [%]	100.0	100
Redundancy	11.39 *	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	19 *	pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	10 (mM)	pH7.5
2	1	drop	ammonium sulfate	100 (%sat)
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	ammonium sulfate	58 (%sat)
5	1	reservoir	Tris-HCl	50 (mM)	pH8.0