1HKF
The three dimensional structure of NK cell receptor Nkp44, a triggering partner in natural cytotoxicity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 60.437, 60.437, 197.253 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 100.000 - 2.210 * |
R-factor | 0.2399 |
Rwork | 0.240 |
R-free | 0.24500 * |
Structure solution method | SIRAS |
RMSD bond length | 0.018 * |
RMSD bond angle | 1.860 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.210 * | 2.210 * |
Rmerge | 0.074 | 0.523 |
Total number of observations | 353554 * | |
Number of reflections | 11487 | |
<I/σ(I)> | 23.6 | 5.7 |
Completeness [%] | 99.6 | 96.8 |
Redundancy | 31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | 278 * | pH 5.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9.6 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 30 (%(v/v)) | |
3 | 1 | reservoir | ammonium acetate | 0.1 (M) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |