1HKF

The three dimensional structure of NK cell receptor Nkp44, a triggering partner in natural cytotoxicity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Spacegroup name	P 62 2 2
Unit cell lengths	60.437, 60.437, 197.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 - 2.210 *
R-factor	0.2399
Rwork	0.240
R-free	0.24500 *
Structure solution method	SIRAS
RMSD bond length	0.018 *
RMSD bond angle	1.860 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.210 *	2.210 *
Rmerge	0.074	0.523
Total number of observations	353554 *
Number of reflections	11487
<I/σ(I)>	23.6	5.7
Completeness [%]	99.6	96.8
Redundancy	31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5	278 *	pH 5.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.6 (mg/ml)
2	1	reservoir	PEG400	30 (%(v/v))
3	1	reservoir	ammonium acetate	0.1 (M)
4	1	reservoir	sodium acetate	0.1 (M)	pH5.0