1HKD
Structure of pea lectin in complex with alpha-methyl-D-glucopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.793, 61.235, 136.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.950 - 2.090 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ltn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.305 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.420 |
| Number of reflections | 30005 | |
| <I/σ(I)> | 17.9 | 1.92 |
| Completeness [%] | 95.4 | 83.5 |
| Redundancy | 2.8 | 2.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.7 M AMMONIUM SULFATE, 10% (V/V) ETHANOL, pH 7.00 |
