1HK7
Middle Domain of HSP90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 112.879, 112.879, 112.059 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 * - 2.500 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.24700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 * | 0.379 * |
| Number of reflections | 28094 | |
| <I/σ(I)> | 4.8 | 1.8 |
| Completeness [%] | 99.9 | 99.9 * |
| Redundancy | 3.6 * | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | 14 * | CRYSTALS GROWN USING MICROBATCH METHOD BY MIXING 1UL OF 24MG/ML,PROTEIN IN BUFFER (20MM TRISHCL),PH 7.5, 1MM EDTA, 0.5MM DTT) WITH 1UL OF 11MM CDSO4, 20MM MGCL2, 80MM TRIS HCL, PH 7.5 AND 5% GLYCEROL. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 24 (mg/ml) | |
| 2 | 1 | 1 | 11 (mM) | ||
| 3 | 1 | 1 | 20 (mM) | ||
| 4 | 1 | 1 | Tris-HCl | 80 (mM) | pH7.5 |
| 5 | 1 | 1 | glycerol | 5 (%(v/v)) |
