1HK7

Middle Domain of HSP90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-15
Detector	ADSC CCD
Spacegroup name	P 61
Unit cell lengths	112.879, 112.879, 112.059
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.600 * - 2.500
R-factor	0.24
Rwork	0.240
R-free	0.24700 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.630
High resolution limit [Å]	2.500	2.500
Rmerge	0.088 *	0.379 *
Number of reflections	28094
<I/σ(I)>	4.8	1.8
Completeness [%]	99.9	99.9 *
Redundancy	3.6 *	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	14 *	CRYSTALS GROWN USING MICROBATCH METHOD BY MIXING 1UL OF 24MG/ML,PROTEIN IN BUFFER (20MM TRISHCL),PH 7.5, 1MM EDTA, 0.5MM DTT) WITH 1UL OF 11MM CDSO4, 20MM MGCL2, 80MM TRIS HCL, PH 7.5 AND 5% GLYCEROL.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	24 (mg/ml)
2	1	1		11 (mM)
3	1	1		20 (mM)
4	1	1	Tris-HCl	80 (mM)	pH7.5
5	1	1	glycerol	5 (%(v/v))